Restriction endonuclease-mediated real-time digestion-PCR for somatic mutation detection
نویسندگان
چکیده
منابع مشابه
Methylation analysis by restriction endonuclease digestion and real-time PCR.
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The polymerase chain reaction (PCR) is the most widely used technique for the study of DNA. Applications for PCR have been extended significantly by the development of "long" PCR, a technique that makes it possible to amplify DNA fragments up to 40 kb in length. This article describes two novel applications of the long PCR technique, one which simplifies restriction mapping and another which en...
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ژورنال
عنوان ژورنال: International Journal of Cancer
سال: 2012
ISSN: 0020-7136
DOI: 10.1002/ijc.27968